Where is thyroid releasing hormone
In some studies, hypothyroidism induced only a moderate increase in D2 mRNA in the hypothalamus and no increase in D2 activity In the same way, when hypothalamic cells were analyzed in association with iodine deficiency, there was no increase in D2 activity in the hypothalamus contrary to what was observed in other regions in the brain Taken together, these studies suggest that maintenance of a constant T 3 tissue level may not be the main function of D2 in the hypothalamus.
In contrast, T 3 produced by tanycytes, a unique glial cell type that lines the third ventricle, may be the primary source of T 3 for feedback regulation of TRH neurons. T 3 can then diffuse into the substance of the brain to reach the hypothalamic PVN 55 or may be released into the median eminence and transported by axon terminals to the hypophysiotropic TRH neurons 24 , 56 — The D2 activity in the tanycytes under different circulating TH levels seems to contribute to the negative feedback regulation of the HPT axis perhaps because it allows the hypophysiotropic TRH neurons to sense any changes in T 4 output by the thyroid gland.
These animals have low brain T 3 levels associated with elevated serum T 4 and TSH levels, indicating the presence of central resistance to TH due to inadequate central production of T 3 As noted above, circulating T 4 is converted to T 3 by D2 in tanycytes. Clearly, the basal level of TRH gene expression is important in determining the set-point for regulation by TH through either a direct or indirect mechanism.
What determines the basal level of TRH gene transcription is a matter of much debate. Moreover, it does not appear to be possible to dissociate T 3 -independent properties of the TR, which activate genes like TRH, from its T 3 -dependent activities that result in TH inhibition of gene expression.
Deletional analysis of the TRH gene identified a region in the proximal promoter, named site 4, that contained two structurally different negative TH response elements. This region is highly conserved both in murine and human species 2 , Although this site is important for cAMP and T 3 regulation of the TRH gene in vitro , the physiological importance of this region in vivo has yet to be proven. Using transgenic knock-in mouse models, the mechanism of TH negative regulation has begun to be explored in vivo.
A second transgenic knock-in mouse model was constructed to determine whether TR cofactor interactions were essential for TH negative regulation. In vitro studies have demonstrated that TR activity is regulated by binding to both corepressor CoR and coactivator CoA proteins on TH positively regulated genes. The physiological role of CoAs bound to TRs, however, had yet to be defined in vivo.
As expected, mice bearing this allelic mutation displayed abnormal TH-stimulated gene expression. Interestingly, however, these animals also displayed abnormal regulation of the HPT axis. These data illustrate the importance of an intact CoA-binding surface for both positive and negative regulation by TH in vivo CoAs are proteins that can remodel chromatin via enzymatic acetylation of histone tails or via regulation of transcriptional complex assembly at the promoter by interactions with RNA polymerase and general transcription factors One of the best studied CoA proteins is Src-1, a member of the p class of transcriptional factors, first identified as steroid receptor coactivator 70 and then characterized as a coactivator of other nuclear receptors, including the TR 71 , Perhaps binding of this cofactor to the TR AF-2 domain recruits other corepressor proteins to the transcriptional complex, which then repress gene expression.
In conclusion, the mechanism of TH negative feedback control of the HPT axis has been clarified by recent studies. Experimental models demonstrated the critical role of hypothalamic TRH in the control of HPT axis and in establishing the set-point of the axis. Further studies are directed at understanding other unique features of TH inhibition at the transcriptional level. Finally, TRH gene expression is also regulated by temperature, food intake, and stress.
Thus the TRH neuron is well positioned to integrate information about the environment as well as circulating TH levels and ultimately affect metabolism in response to these physiological changes. J Biol Chem : — Google Scholar. Mol Endocrinol 9 : — Mol Cell Endocrinol 94 : — Mol Endocrinol 7 : — Endocrinology : 92 — Mol Cell Biol 15 : — Tagami T , Madison LD , Nagaya T , Jameson JL Nuclear receptor corepressors activate rather than suppress basal transcription of genes that are negatively regulated by thyroid hormone.
Mol Cell Biol 17 : — J Biol Chem 5 : — Science : 78 — Persani L Hypothalamic thyrotropin-releasing hormone and thyrotropin biological activity. Thyroid 10 : — Endocr Rev 14 : — J Clin Invest : — J Clin Invest 61 : — Science : — Endocrinology : — Fekete C , Lechan RM Negative feedback regulation of hypophysiotropic thyrotropin-releasing hormone TRH synthesizing neurons: role of neuronal afferents and type 2 deiodinase.
Front Neuroendocrinol 28 : 97 — Neuroendocrinology 47 : — Immunocytochemistry combined with retrograde labeling at the light and electron microscopic levels. Acta Biol Hung 45 : — J Neurosci 20 : — Annu Rev Neurosci 6 : — Neurosci Lett : — Neuroendocrinology 64 : — Biochem J : 73 — Evidence for direct and indirect pathways. Wittmann G , Liposits Z , Lechan RM , Fekete C Medullary adrenergic neurons contribute to the neuropeptide Y-ergic innervation of hypophysiotropic thyrotropin-releasing hormone-synthesizing neurons in the rat.
Neurosci Lett : 69 — Wittmann G , Liposits Z , Lechan RM , Fekete C Medullary adrenergic neurons contribute to the cocaine- and amphetamine-regulated transcript-immunoreactive innervation of thyrotropin-releasing hormone synthesizing neurons in the hypothalamic paraventricular nucleus.
Brain Res : 1 — 7. Acta Physiol Scand : — Prog Brain Res : — Curr Opin Endocrinol Diabetes 3 : — De Groot LJ Dangerous dogmas in medicine: the nonthyroidal illness syndrome. J Clin Endocrinol Metab 84 : — Mihaly E , Fekete C , Legradi G , Lechan RM Hypothalamic dorsomedial nucleus neurons innervate thyrotropin-releasing hormone-synthesizing neurons in the paraventricular nucleus. Brain Res : 20 — J Comp Neurol : — For instance, an injection of thyrotropin-releasing hormone has effects on the arousal and feeding centres of the brain, causing wakefulness and loss of appetite.
As its name implies, the main effect of thyrotropin-releasing hormone is to stimulate the release of thyrotropin also known as thyroid stimulating hormone from the pituitary gland.
Thyrotropin-releasing hormone is the master regulator of thyroid gland growth and function including the secretion of the thyroid hormones thyroxine and triiodothyronine. If there is insufficient thyroid hormone available for the brain, this will be detected by the hypothalamus and thyrotropin-releasing hormone will be released into the blood supplying the pituitary gland. The effect of thyrotropin-releasing hormone on the pituitary gland is to trigger thyroid stimulating hormone release, which, in turn stimulates the thyroid gland to make more thyroid hormone.
Thyrotropin-releasing hormone in its pharmaceutical formulation of 'protirelin' was widely used as a drug to test whether someone had thyroid overactivity. However, there are now more sensitive measurements that can detect very low levels of thyroid stimulating hormone in the blood. Thyrotropin-releasing hormone tests are still occasionally carried out but are normally used for the diagnosis of conditions caused by resistance to thyroid hormone action.
If a person has too little thyrotropin-releasing hormone, they will develop thyroid underactivity hypothyroidism. This is a rare condition, usually due to an injury or tumour which destroys this area of the hypothalamus. This situation is referred to as secondary or central hypothyroidism.
About Contact Events News. Search Search. Additional information about TSH and its effects and control are presented in the section on the thyroid gland. Growth Hormone. Adrenocorticotrophic Hormone. Updated Send comments to Richard.
0コメント